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偶联EGFR单抗C225的Fe3O4/Ag复合纳米颗粒的抗体活性鉴定及其对鼻咽癌细胞增殖的作用 |
[高血压医学论文]
作者：赵迪，孙新臣，马珺，童金龙，徐睿智，张伟，肖韦华
【摘要】 目的：研究多功能纳米材料Fe3O4/Ag/C225在体外的稳定性、靶向性及抗肿瘤疗效。方法:检测复合纳米颗粒Fe3O4/Ag的释药水平;通过MTT比色法初步检测Fe3O4/Ag/C225抑制鼻咽癌肿瘤细胞生长的作用;采用ELISA法检测复合纳米颗粒Fe3O4/Ag/C225上的C225的稳定性。结果:复合纳米颗粒Fe3O4/Ag/C225释药缓慢;偶联在复合纳米颗粒Fe3O4/Ag/C225上C225保持了抗体本身约82%的活性;复合纳米颗粒对鼻咽癌肿瘤细胞抑制作用明显。结论:Fe3O4/Ag/C225在体外稳定性好，偶联C225的复合纳米颗粒保存了抗体活性，对鼻咽癌肿瘤细胞有抑制作用。Fe3O4/Ag/C225有望发展成为分子影像调强放疗的多功能示踪剂。
【关键词】 Fe3O4/Ag/C225; 纳米; 鼻咽癌
[Abstract] Objective: To study the stability, targeting and antitumor effect of multifunctional nanoparticles Fe3O4/Ag/C225 in vitro. Methods: The release ratio of Fe3O4/Ag/C225 was tested. Inhibitory effect of Fe3O4/Ag/C225 on nasopharyngeal carcinoma tumor cell growth was detected by MTT assay. The level of preserved antibody activity in the composite nanoparticles coupled C225 was detected by ELISA. Results: Composite nanoparticles released slowly. Experiments showed that composite nanoparticles inhibited tumor cells of nasopharyngeal carcinoma obviously.The C225 antibody coupling the composite nanoparticles Fe3O4/Ag was mai……
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最后编辑：admin888 |
高血压医学论文 | 高血压医学论文 |
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