作者：刘锐，黄沙，金岩，吴红，姜玲，刘涛

　【关键词】  ,碱性成纤维细胞生长因子

    Preparation and characteristic test of bFGFimpregnated microsphere

　　【Abstract】 AIM: To prepare the basic fibroblast growth factor (bFGF)impregnated microsphere and to study its characteristics. METHODS:  The bFGFimpregnated microsphere was prepared by improved emulsified coldcondensation method. The physical properties, drug content and encapsulation were detected. The drug release in vitro was measured by ELISA. RESULTS:  The average diameter of microsphere was (12.4±3.6) μm, the average drug content was 0.24% and encapsulation was 96.82%. In vitro, 28.23% of bFGF was sustainedreleased on the first day, 57.19% from the second to the fifth day and 93.94% by the seventh day. CONCLUSION:  The preparation method is simple, rapid and accurate and the release of bFGF from the microspheres can be sustained for a longer time.

　　【Keywords】 basic fibroblast growth factor; microsphere; sustainedrelease; encapsulation

　　【摘要】 目的：制备复合碱性成纤维细胞生长因子（bFGF）的可降解缓释微球，考察其各项性能，为进一步 应用 于组织工程奠定基础. 方法 ：采用改良的乳化冷凝法交联制备复合bFGF的缓释微球，测定微球形态、粒径、载药率和包裹率等，并采用ELISA法考察其体外bFGF的缓释效能. 结果：缓释微球平均粒径(12.4±3.6) μm；平均载药率为0.24%，平均包裹率为96.82%.  d 1体外释放28.23%，2~5 d时累积释放率达到57.19%, d 7时释放减慢且累计……