【摘要】 白血病细胞异常免疫表型是区别于正常造血细胞的重要特征之一，也是流式细胞术检测微小残留病的基础。为了了解急性白血病的异常免疫表型特征，本研究采用四色流式细胞术CD45/SSC双参数散点图设门技术对126例急性白血病患者的异常免疫表型进行分析，并初步探讨其在微小残留病检测中的意义。结果显示：约76％的患者可以检测到明确的异常抗原表达，白血病异常免疫表型可分为以下四类：抗原跨系列表达、跨阶段表达、过度表达及缺失表达，其阳性率分别为39％、46％、21％和29％。约11％的患者仅发生了单一的表型异常，其余患者则可以检测到两类或更多的表型异常。结论：在大多数急性白血病患者中可以检测到明确的白血病异常免疫表型，在此基础上应用多参数流式细胞仪可以有效检测微小残留病变。
【关键词】 急性白血病
Aberrant Immunophenotypes in 126 Patients with Acute Leukemia
Abstract The existence of leukemia aberrant immunophenotypes (LAIP) has been suggested to be a valuable tool for the detection of minimal residual disease (MRD), as they could distinguish leukemic cells from normal hematopoietic progenitors. This study was purposed to analyze the characteristics of LAIP in acute leukemia and further explore the proportion of different types of LAIP in acute leukemia patients. Flow cytometry (FCM) with four color and CD45/SSC gating were used to detect the antigen expression in samples of bone marrow from 126 patients with acute leukemia.&nb……
<<<<<全文未完，本文约4032个中文字，未计算英文字母、数字>>>>>
