作者：林旭初　惠延年　孟浩　张勇杰　金岩

【摘要】  　　目的：制备低抗原性、保留天然角膜结构的组织工程角膜支架材料。方法：对猪角膜进行反复冻融联合酶消化，最后冷冻干燥，观察其组织学特征。结果：制备的脱细胞猪角膜基质的细胞成分能被有效去除，材料保留了利于角膜上皮生长的基底膜和有序排列的网状的基质胶原结构，有一定的强度和韧性。结论：采用反复冻融联合酶消化、冷冻干燥法能获得一种无细胞的组织工程角膜支架材料。

【关键词】  生物衍生材料　脱细胞角膜基质　组织工程

　　Preparation and observation of the histological structure of the acellular porcine corneal stroma

       Abstract AIM: To prepare the scaffold of the tissue engineering cornea with the lower antigen and native structure of corneal stroma.METHODS: The acellular porcine corneal stroma (APCS) was prepared by the means of enzymatic digestion, freezing and thawing and lyophilization, and the histological structure of APCS was observed. RESULTS: The cells in the porcine corneal stroma could be removed effectively. The APCS retained the basement membrane. The structure of stromal collagen was ordered and tough. CONCLUSION: The APCS prepared by the means of enzymatic digestion, freezing and thawing and lyophilization is a kind of tissue engineering corneal scaffold derived from biologic materials. It has the character of the lower antigen and possessing the native structure of the corneal stroma to facilitate the growth of the seed cells.

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