[生化制药论文]
作者：付建武，彭红，周玉春，黄丽芸
【摘要】 目的采用HPLC波长转换法测定栀子药材中西红花苷-1和栀子苷的含量。方法色谱柱为Agilent Zorbax Eclipse XBD-C18，流动相为乙腈∶水(梯度洗脱) ，流速为1 ml/min，检测波长为238 nm（0～20 min）;440 nm（20～40 min），柱温为30℃,采用20 min转换检测波长。结果栀子苷在4.52～180.8 μg范围内, r=0.999 9，西红花苷-1在0.9～9.0 μg范围内，r=0.999 7，与色谱峰面积呈线性关系。平均回收率分别为99.52％，100.76%,RSD分别为1.10%，1.41%。结论该测定方法具有简便、稳定、准确、重复性好的特点。
【关键词】 高效液相法； 栀子苷； 西红花苷-1
Abstract：ObjectiveTo determine the jasminoidin and safflower glucoside-1 of Gardenia. MethodsUsing RE-HPLC with Agient Zorbax Eclipse XBD-C18 column,the mobile phase was composed of a mixture of acetonitrile-water(grads elution).The flow rate was 1ml/min, and the UV detection wavelengths were set at 238nm(0～20 min)and 440nm(20～40 min),and the tempetature was at 30℃.The wavelength was converted at 20min.ResultsIrisflorentins of the drugs showed linear relationship with peak areas in the range of 4.52～180 μg/ml and 0.9～9.0μg/ml respectively(r=0.999 9,0.999 7).The average recoveries were 99.52% and 100.76%.The RSD were 1.10% and 1.41%.ConclusionThe method is simple,stable,accurate and repea……
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