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高效液相色谱内标法测定新疆各地鲜蒜中蒜氨酸的含量 |
[生化制药论文]
作者：唐辉，陈坚，王鲁石，景文娟，艾波
【摘要】 目的调查新疆各地鲜蒜中蒜氨酸含量，评价鲜蒜品质，判断药用大蒜来源。方法对地区多点采样，以微波方式灭酶并采用高效液相色谱内标法进行鲜蒜中蒜氨酸含量测定。结果新疆各地大蒜中蒜氨酸含量范围从0.657%～2.474%。结论新疆大蒜蒜氨酸含量普遍较高，差异较大。
【关键词】 新疆鲜蒜 蒜氨酸
Abstract:ObjectiveTo investigate the alliin content from fresh garlic in Xinjiang and to evaluate the quality of fresh garlic so as to estimate the source of garlic for pharmaceutical application. MethodsTo collect samples from various spots in different areas. After an disposal of microwave for allinase deactivation，alliin in fresh garlic was determined by HPLC with internal standard substance. ResultsThe content range of alliin was 0.657%～2.474%. ConclusionThe contents of alliin in garlic of Xinjiang are universally high, but the differences are relatively remarkable.
Key words:HPLC with internal standard ； Alliin
百合科葱属植物大蒜Allium Sativum Linn为多年生草本植物。大蒜作为药用植物，除抗菌、消炎外，尤其对于几种癌症和心脏血管失调具有特殊预防和治疗作用［1］。中医典藉(唐)《新修本草》、(元)《食物本……
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