作者：杨立芳， 李小燕， 罗伟强， 潘洪平，邓光辉, 韦冬萍

【关键词】  广西粉葛；,,葛根素；,,高效液相色谱法

　　摘要：目的采用HPLC法测定广西粉葛中葛根素的含量。方法色谱柱Phenomenex Gemini C18柱（250 mm ×4.6 mm, 5 μm）；流动相：乙腈(A)-0.4%磷酸水溶液(B)，采用梯度洗脱；流速：1.0 ml/min；检测波长：250 nm；柱温：30℃。结果葛根素在800~2400 ng范围内峰面积与进样量呈良好的线性关系。回归方程为A=7.682×104C +5.885×104，r=0.999 3（n=5）。其平均回收率为99.49％，RSD为0.75％(n=5)。结论该方法简便易行，结果准确，可作为广西粉葛药材质量控制的有效方法。

　　关键词：广西粉葛；  葛根素；  高效液相色谱法

　　Determination of Puerarinin Content in Guangxi Radix puerariae thomsonii by HPLC

　　Abstract：ObjectiveTo determine the content of puerarin from Guangxi Radix puerariae thomsonii by HPLC.Methods The isolation was conducted on a phenomenex gemini C18 column (250 rmm ×4.6 mm , 5 μm ), the mobile phase consisted of acetonitnle (A)-0.4% phosphoric and (B) in a gradient model, the flow rate wes 1.0 ml/min, the detection wavelength was 250 nm and the column temporature was 30℃.ResultsThe content of puerarin showed good linearity in the range of 800 ng~2400 ng，the regression equation was A=7.682×104C +5.885×104，r=0.999 3 (n=5), and the average recovery was 99.49％, RSD was 0.75% (n=5).ConclusionThis method is simple, rapid, accurate and reliable. It can be used for quality control of Guangxi Radix puerariae thomsonii.

　　Key word……