作者：王利胜 占建坤 周莉玲

【关键词】  高效液相色谱

　　摘要：目的建立跌打活络微乳中蛇床子素含量的测定方法。方法采用高效液相色谱法，色谱柱：Diamonsil钻石C18（5 μm，250 mm×4.6 mm）；流动相：甲醇水（80∶20），检测波长：322 nm；流速：1 ml/min。结果蛇床子素在0.042~ 0.336 μg线性关系良好，r=0.999 7，平均回收率为100.91%，RSD为2.42%(n=6)。结论该法操作简便，结果准确，灵敏度高，重现性好，可用于该制剂的质量控制。

　　关键词：跌打活络微乳；高效液相色谱法；蛇床子素
                        
　　Determination of Osthole in Dieda Huoluo Microemulsions by HPLC

　　Abstract：ObjectiveTo establish the determination method for osthole in Dieda Huoluo Microemulsions. MethodsHPLC was used with DiamonsilC18 column (250 mm×4.6 mm, 5 μm), detection wavelength at 322 nm, mobile phase: methanol-water (80∶20), flow rate: 1ml/min. ResultsOsthole has good linear relationship between 0.042~0.336 μg, r=0.999 7, the recovery of osthole was 100.91% and RSD was 2.42% (n=6). ConclusionThe method is simple, sensitive and reliable to carry out to control the quality of this preparation.

　　Key words：Dieda Huoluo Microemulsions; HPLC; Osthole

　　跌打活络膏是由蛇床子、红花、大黄等几味中药材制成，具有活血化瘀、消肿止痛、收敛生肌、舒筋活络的功效，临床用于治疗跌打损伤、水火伤烫伤、扭伤、挫伤、瘀血疼痛等疗效显著。为了提……