[高血压医学论文] 关键词: 激光;共聚焦显微镜;粘附细胞分析与筛选(ACAS)
摘 要 激光扫描共聚焦显微镜是近十年发展起来的医学图象分析仪器,现已广泛应用于荧光定量测量、共焦图象分析、三维图象重建、活细胞动力学参数监测和胞间通讯研究等方面。其性能为普遍光学显微镜质的飞跃,是电子显微镜的一个补充。本文以美国Meridian公司的ACAS ULTIMA312为例简要介绍了激光扫描共聚显微镜系统的结构,功能和生物学应用前景。
关键词 激光;共聚焦显微镜;粘附细胞分析与筛选(ACAS)
The Laser Scanning Confocal Microscopy System and its Biological Applications
Chen Yaowen ,Lin Jielong ,Lai Xiaoying ,Mei Pinchao
(Shantou Uni.Med .College,Central Lab ,Shantou Guangdong 515031)
Ahstract The Laser Scanning Confocal Microscopy is a new medical image analysis instrument ,which is developed in the last decade.Now it is widely applied in such fields as fluorescent quantitative measurement ,conpocal image andlyusis ,3-D reconstruction ,Kinetic signal monitioring of living cell ,cellcell communication researches ,etc .In this paper ,ACSA ULTIMA 312(Meridian Co,USA)is taken as an example to introduce the principle of confocal microscopy ,its funetions and biological applications.
Key words Laser Confocal Microscopy Adherent Cell Analysis and sorting(ACSA)
激光扫描共聚焦显微镜(Laser scanning Confocal Microscopy ,简称LSCM)是近代生物医学图象仪器的最重要发展之一,它是在荧光显微镜成象的基础上加装激光扫描装置,使用紫外光或可见光激发荧光探针,利用计算机进行图象处理,从而得到细胞或组织内部微细结构的荧光图象,以及在亚细胞水平上观察诸如Ca2+、pH值、膜电位等生理信号及细胞形态的变化。已广泛应用于细胞……
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