【摘要】  目的 建立普卢利沙星的含量测定方法。方法 采用高效液相色谱法；Alltima C18柱(150mm×4.6mm，5μm)；乙腈∶0.005mol/L磷酸二氢钠溶液(取0.005mol/L磷酸二氢钠溶液100ml，加三乙胺0.2ml，用磷酸调节pH值至6.0)(40∶60)为流动相；流速1.0ml/min，检测波长278nm。结果 普卢利沙星在12.8～204.1μg/ml浓度范围内呈现良好的线性关系，r=0.9999。结论 该方法快速、简捷、准确都高，适用于普卢利沙星的含量测定。

【关键词】  普卢利沙星 含量测定 HPLC法

    Content determination of prulixacin by HPLC

      ABSTRACT  Objective  A high performance liquid chromatographic method for the content determination of prulixacin was developed.  Method  The HPLC was performed on a column of Alltima C18 (150mm×4.6mm, 5μm), using the mobile phase of 0.005mol/L sodium dihydrogen phosphate (added with 0.2ml triethylamine and adjusted to pH 6.0 by phosphoric acid)∶acetonitrile (60∶40) at the detection wavelength of 278nm with the flow rate of 1.0ml/min.  Results  The calibration curve was linear in the range of 12.8～204.1μg/ml (r=0.9999) for prulixacin.  Conclusion  The method was simple, quick and accurate for content determination of prulixacin.

    KEY WORDS  Prulixacin;  Content determination;  HPLC

     普卢利沙星是由日本新药公司与明治制果公司共同开发研制的第三代氟喹诺酮类广谱抗菌药物，对革兰阳性菌和革兰阴性菌有广谱抗菌作用，对铜绿假单胞菌的MIC90为0.39μg/ml，远超过其他同类产品的抗菌活……