作者：李美山，张成，陈松林，于美娟，张雅妮，王淑辉，熊符

【关键词】  腺病毒

    Coexpression of both green fluorescent protein and myogenic regulatory factors in bone marrowderived mesenchymal stem cells

　　【Abstract】 AIM: To investigate the transfection of bone marrowderived mesenchymal stem cells (MSCs) by recombinant adenovirus (Ad), and the expressions of both green fluorescent protein (GFP) and myogenic regulatory factors, which are MyoD and Myogenin. METHODS: Rat MSCs were separated, cultured and expanded in vitro, and taken for identification of surface antigens by flow cytometry (FCM). AdGFP was amplified and identified to transfect MSCs. Expressions of both MyoD and Myogenin were detected in transfected MSCs by RTPCR. Differentiation of MSCs was induced by cocultured with C2C12 myoblasts, and MyoD expression was identified by immunofluorescence (IF). RESULTS: FCM showed that CD11b and CD45 were negative, CD29 and CD44 were positive in MSCs surface antigens. With the increasing of AdGFP multiple of infection (MOI), transfection rate were also increased. When MOI was over 100, cytopathic effect appears on MSCs. Expressions of both MyoD and Myogenin in transfected MSCs were approved by RTPCR; and MyoD protein can be found in induced MSCs by IF. CONCLUSION: MSCs can be effectively transfected by AdGFP and express GFP; and activation of intrinsic myogenic regulatory factors will enhance the myogenic differentiation of MSCs……