【关键词】  外胚间充质干细胞

　　Expression of human telomerase in ectomesenchymal stem cells of early embryonic facial process

　　【Abstract】 AIM:  To evaluate the activity of telomerase in ectomesenchymal stem cells of early embryonic facial process. METHODS:  Ectomesenchymal stem cells of early human embryonic facial process were isolated and cultured in undifferentiated conditions by supplementing with leukemia inhibitor factor (LIF). Immunohistochemistry assay and image analysis were used to detect the expression extent of human telomerase reverse transcriptase (hTERT) when cells were passaged for 1, 8, 15 and 22 times. Cells without LIF for 10 days were also evaluated.  RESULTS: Ectomesenchymal stem cells expressed hTERT. The expression extent of hTERT decreased with the increasing times of passages. The differentiated cells didn't express hTERT. CONCLUSION:  Though ectomesenchymal stem cells of early embryonic facial process have somewhat high telomerase activity, the telomerase activity at this level is not enough to prevent cells from senescence.

　　【Keywords】 ectomesenchymal stem cell； human telomerase reverse transcriptase

　　【摘要】  目的： 探讨面突外胚间充质干细胞的端粒酶活性状况. 方法 ： 体外培养妊娠50 d人胚胎面突外胚间充质干细胞，白血病抑制因子（LIF）抑制细胞分化，于第1, 8, 15和第22代免疫组化检测人端粒……