作者：王晓燕　刘兵　要晖宇　侯宁　杨晓　于晓妉　毛宁

【摘要】  　　为了探讨Ras信号通路对小鼠胚胎干细胞(Embryonic Stem cells, ES cells)造血分化的 影响 ，将突变型基因RasN17转染小鼠ES细胞，免疫印迹检测RasN17的表达对Erk1/2及Akt磷酸化的作用， 应用 半定量RTPCR检测ES细胞在分化过程中与造血相关的基因的表达。结果表明：Ras N17的表达能抑制Erk1/2和Akt的磷酸化，携带RasN17基因的ES细胞在分化过程中Runx1 、SCL 及betamajor珠蛋白等基因的表达被显著抑制， 而FLK1的表达不受影响。结论：ES细胞体外造血分化需要Ras通路的活化。

【关键词】  胚胎干细胞； 造血分化； Ras

　　 Suppression of Ras Mediated Signaling Attenuates Hematopoietic Differentiation of Embryonic Stem Cells  of Mice In Vitro

        Abstract    To investigate the possible involvement of Ras signaling in the hematopoietic differentiation of embryonic stem cells (ES cells), ES cells were transfected with RasN17, the dominantnegative mutant of Ras.  Western blot was used to test the effect of RasN17 expression on Erk1/2 and Akt phosphorylation, semiquantitative RTPCR was used to detect expression of gene related to hemalopoiesis in differentiation of ES cells. The   results showed that the expression of RasN17 in the ES cells remarkably downregulated the phosphorylation of Erk1/2 and Akt simultaneously.  Moreover,  the expression of several markers related with hematopoiesis including Ru……

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