【摘要】 To quantitatively detect gene expression level of transforming growth factor-β type 1 receptor (TβR 1) and transforming growth factor-β type 2 receptor (TβR 2) in different stage of diabetic rats’ retina. To observe and analyze the effect of transforming growth factor-β receptors on the retina of rat diabetic animal model. METHODS: 28 healthy adult Sprague-Dawley rats were chosen and randomly divided into two groups of normal control (CON) and diabetes mellitus (DM). Diabetes was induced by streptozotocin (STZ) intraperitoneal injection. Gene expression was detected quantitatively with real-time fluorescence quantitative reverse transcription polymerase chain reaction (QRT-PCR). RESULTS: The mRNA level of TβR 1 and TβR 2 was 0.000493±0.000133 and 0.000166±0.000057 at 4wk. The mRNA level of TβR 1 and TβR 2 was 0.000608±0.000232 and 0.000113±0.000049 at 12wk. TβR 1 expression was gradually elevated during the progression of diabetic retinopathy, TβR 2 expression was up-regulated at 4wk, but down-regulated at 12wk. CONCLUSION: TGF-βand its receptors (TβR 1 and TβR 2) may play important role in the pathogenesis of diabetic retinopathy.
【关键词】 diabetic retinopathy
INTRODUCTION
Diabetic retinopathy (DR) is the main complication of diabetes ……
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