论文目录 | |
| CHAPTER I Introduction to the model organism Caenorhabditis elegans | 第12-18
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| Origins of the model | 第13
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| Powerful biology model | 第13-14
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| Genome | 第14-15
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| Genetics | 第15-16
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| Neurobiology | 第16-18
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| CHAPTER IICaenorhabditis elegans sister chromatid cohesion proteinsEVL-14/PDS-5 and SCC-3 are required for both meiosis and mitosis | 第18-53
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| Introduction | 第19-23
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| ResultsMutations in evl-14 and scc-3 disrupted the development ofthe vulva and gonad | 第23-29
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| evl-14 and scc-3 encode the likely homologs of the yeast Pds5 and Scc3respectively | 第29-38
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| Loss of evl-14 and scc-3 gene functions disrupt sister chromatid cohesionduring meiosis | 第38-45
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| The localization of REC-8 to chromosomes is completely disruptedin scc-3 mutants | 第45-48
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| scc-3 RNAi results in embryonic lethalityand evl-14/pds-5 RNAi causes a range of phenotypes | 第48-52
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| evl-14/pds-5 is expressed in embryo and postembryonic dividing cells | 第52-53
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| DiscussionPvl Ste mutants primarily represent genes involved in cell divisionand cell cycle regulation | 第53-60
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| EVL-14/PDS-5 and SCC-3 function in both mitosis and meiosis | 第53-54
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| SCC-3 is essential for sister chromatid cohesion | 第54-57
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| EVL-14/PDS-5 is important for maintaining sister chromatid cohesionin late prophase | 第57-58
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| Model of cohesin | 第58-60
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| Materials and methods | 第60-65
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| C.e;egams Strains | 第60
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| Genetic mapping and molecular cloning | 第60-61
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| Double-stranded RNAi | 第61-62
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| Gonad preparation and immunostaining | 第62
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| DAPI staining and FISH | 第62-63
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| Microscopy | 第63-64
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| GFP reporter construction | 第64-65
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| ACKNOWLEDGMENTS | 第65-66
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| REFERENCES | 第66-70
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| PUBLICATIONS | 第70-71
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